In Situ Sequencing

In Situ sequencing is typically a sub-cellular, targeted spatial approach to gene identification and localization within tissues. Often used as a validation method following single cell and spatial transcriptomics studies, it allows researchers to perform “real time” image capture of RNA transcripts within a cell.

Single cell sequencing and spatial transcriptomics are typically exploratory approaches which yield whole transcriptome coverage using a sequencing output. While thousands of transcripts may be present in a cell, few are typically involved in pathways relevant to the disease of interest. In Situ sequencing allows for the design of a targeted probe panel to relevant genes. Because probe hybridization (and corresponding fluorescence output) is imaged directly on the tissue, there is no need for RNA extraction, cell encapsulation, or sequencing output. Improved resolution, sensitivity and specificity allows for the identification of rare transcripts across tissues or within distinct cell types.

10x Genomics Xenium

• (2) 12x24mm capture areas per run
• Targeted transcriptional panels- Custom/off the shelf
  • Up to 5,000 plex RNA detection
• Compatible with post-processing Protein detection (Akoya, IF, H&E)
• Fresh frozen, FFPE compatible

Vizgen Merscope

• 1cm² capture area per run
• Targeted transcriptional panels- Custom/off the shelf
  • Up to 1,000 plex RNA detection
• Protein co-detection- up to 6 user selected proteins
• Fresh frozen, FFPE compatible