Spatial Transcriptomics

While Single Cell sequencing allows us to identify the specific transcriptomic profile of an individual cell, it relies on a cell suspension, dissociated from its natural state.  Tissue structure plays a critical role in understanding disease development.  Cell-cell interactions, immune infiltration and the tumor microenvironment are all understood on a spatial level and cannot truly be visualized without spatial context.  

Spatial transcriptomics allows for the capture of RNA from cells in intact tissue sections, allowing for the retention of the tissue structure, coupled with RNA profiling.   As such, not only are we able to understand transcriptional changes, we can also see how they relate to surrounding cell types.  Immune response to disease is the body’s most natural and effective defense mechanism.  Often, however, ineffective signaling prevents the immune system from responding to and destroying tumor cells.  With spatial transcriptomics, the changes in the RNA content which directly impact downstream protein signaling can be elucidated and paired with identification of immune cell infiltration within the tissue.

Technology Highlights

10x Visium CytAssist

  • 6.5mm2 or 11mm2 capture areas- 2 per slide
    • 55um resolution
    • HD 2um resolution available in 6.5mm2
  • Probe based, whole transcriptome approach for human & mouse
  • Protein co-detection – 35plex including intra & extracellular
  • Compatible with Xenium slides
  • Fresh frozen, FFPE compatible

Curio Seeker

  • 3mm2 or 10mm2 capture areas- 1 per slide
  • 10um resolution
  • Unbiased, whole transcriptome analysis
  • Organism agnostic
  • Fresh frozen compatible only

Curio Trekker

  • 10mm2 capture areas- 1 per slide
  • “True” single cell resolution
  • Compatible with 10x & Fluent
  • Unbiased, single nuclei RNA-seq
  • Organism agnostic
  • Fresh frozen compatible only